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Does his absence make a difference to your work?
没有他，会影响你的工作吗？
make a/no/some difference to sb./sth.: have an/no/some effect on sb./sth. 对……有效、无效、有些效果；对……重要、不重要
例句：
Whether he is here or not will make no difference to our work.
他在不在对我们的工作没有影响。
Education can make a big difference to the quality of a person's life.
教育对一个人的生活质量有很大影响。
